LymphedemaV1, Main, Exploration, bibRecord, 00E633

Identification and partial characterization of a parasite antigen in sera from humans infected with Wuchereria bancrofti.

Identifieur interne : 00E633 ( Main/Exploration ); précédent : 00E632; suivant : 00E634

Identification and partial characterization of a parasite antigen in sera from humans infected with Wuchereria bancrofti.

Auteurs : G J Weil ; F. Liftis

Source :

Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1987.

RBID : pubmed:3553330

Descripteurs français

KwdFr :
- Anticorps monoclonaux (immunologie), Antigènes d'helminthe (analyse), Filariose lymphatique (immunologie), Humains, Masse moléculaire, Techniques d'immunoadsorption, Techniques immunoenzymatiques, Wuchereria (immunologie), Wuchereria bancrofti (immunologie).
MESH :
- analyse : Antigènes d'helminthe.
- immunologie : Anticorps monoclonaux, Filariose lymphatique, Wuchereria, Wuchereria bancrofti.
- Humains, Masse moléculaire, Techniques d'immunoadsorption, Techniques immunoenzymatiques.

English descriptors

KwdEn :
- Antibodies, Monoclonal (immunology), Antigens, Helminth (analysis), Elephantiasis, Filarial (immunology), Humans, Immunoenzyme Techniques, Immunosorbent Techniques, Molecular Weight, Wuchereria (immunology), Wuchereria bancrofti (immunology).
MESH :
- chemical , analysis : Antigens, Helminth.
- chemical , immunology : Antibodies, Monoclonal.
- immunology : Elephantiasis, Filarial, Wuchereria, Wuchereria bancrofti.
- Humans, Immunoenzyme Techniques, Immunosorbent Techniques, Molecular Weight.

Abstract

The purpose of this study was to identify and characterize soluble parasite antigens present in sera from humans infected with the filarial nematode Wuchereria bancrofti. Affinity chromatography and immunoblot methods were used to demonstrate a 200,000 m.w. circulating parasite antigen in sera from infected humans which corresponded to an antigen released by adult W. bancrofti during in vitro culture. Two monoclonal antibodies were produced to this antigen by immunizing mice with antigens from Dirofilaria immitis, a filarial parasite that is closely related to W. bancrofti, and screening cell fusion supernatants by enzyme immunoassay and counterimmunoelectrophoresis inhibition. The antibodies bound to a single repeated epitope (not phosphorylcholine) that was resistant to heat, acid, and protease treatments but sensitive to periodate oxidation. Immunoperoxidase studies showed that the epitope was concentrated in the cuticle and reproductive organs in D. immitis, and it was released in relatively large amounts by adult female D. immitis in vitro. The epitope is also present in antigens of other species of filarial and nonfilarial nematodes, but on the basis of preliminary studies, its presence in human serum appears to be specific for W. bancrofti infection.

PubMed: 3553330

Affiliations:

Le document en format XML

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<term>Masse moléculaire</term>
<term>Techniques d'immunoadsorption</term>
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<term>Wuchereria bancrofti</term>
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<term>Immunosorbent Techniques</term>
<term>Molecular Weight</term>
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<front><div type="abstract" xml:lang="en">The purpose of this study was to identify and characterize soluble parasite antigens present in sera from humans infected with the filarial nematode Wuchereria bancrofti. Affinity chromatography and immunoblot methods were used to demonstrate a 200,000 m.w. circulating parasite antigen in sera from infected humans which corresponded to an antigen released by adult W. bancrofti during in vitro culture. Two monoclonal antibodies were produced to this antigen by immunizing mice with antigens from Dirofilaria immitis, a filarial parasite that is closely related to W. bancrofti, and screening cell fusion supernatants by enzyme immunoassay and counterimmunoelectrophoresis inhibition. The antibodies bound to a single repeated epitope (not phosphorylcholine) that was resistant to heat, acid, and protease treatments but sensitive to periodate oxidation. Immunoperoxidase studies showed that the epitope was concentrated in the cuticle and reproductive organs in D. immitis, and it was released in relatively large amounts by adult female D. immitis in vitro. The epitope is also present in antigens of other species of filarial and nonfilarial nematodes, but on the basis of preliminary studies, its presence in human serum appears to be specific for W. bancrofti infection.</div>
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Identification and partial characterization of a parasite antigen in sera from humans infected with Wuchereria bancrofti.

Identification and partial characterization of a parasite antigen in sera from humans infected with Wuchereria bancrofti.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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